Characterization of a Wheat-Dasypyrum breviaristatum Chromosome Addition and Its Derived Progenies Carrying Novel Dasypyrum-Specific Gliadin Genes
نویسندگان
چکیده
The construction of the 28-chromosome karyotype Dasypyrum breviaristatum was undertaken using multicolor non-denaturing fluorescent in situ hybridization (ND-FISH) and Oligo-FISH painting protocols. A novel wheat-D. line D2138 contained 44 chromosomes including a pair D. 6VbS.2VbL translocation chromosomes. Individual F2 F3 progenies cross between with wheat lines CM62, MY11 JM22, respectively, were characterized ND-FISH molecular markers. relatively high chromosome alteration rate within 6VbS 2VbL observed three progeny populations, suggesting that has gametocidal-like gene. different types deletion allowed localization breviaristatum-specific gliadin coding genes on sub-telomeric regions by PCR acid polyacrylamide gel electrophoresis analysis. positive effect agronomic quality characters also demonstrated. new will be useful as germplasm for breeding purposes.
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ژورنال
عنوان ژورنال: Agronomy
سال: 2022
ISSN: ['2156-3276', '0065-4663']
DOI: https://doi.org/10.3390/agronomy12071673